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nitric oxide  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology nitric oxide
    Nitric Oxide, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitric+oxide+no+colorimetric+assay+kit/Nitric+Oxide+(NO)+Colorimetric+Assay+Kit/pmc13088688-89-0-28
    Average 96 stars, based on 185 article reviews
    nitric oxide - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Lactiplantibacillus plantarum LPPerfectus001 Alleviating Irritable Bowel Syndrome in Mice by Modulating Gut Microbiota and NF-κB Signaling Pathway
    Article Snippet: After this pretreatment, 10 μL of a 100 μg/mL LPS (Sigma-Aldrich, Natick, MA, USA) working solution was added, and the cells were incubated for an additional 22 h. After treatment, both the cells and supernatants were collected. .. The concentration of nitric oxide (NO) in the supernatant was measured using Nitric Oxide (NO) Colorimetric Assay Kit (Elabscience, Wuhan, China). .. To determine the expression level of pro- inflammatory genes, total RNA was isolated using AG RNAex Pro reagent and reverse-transcribed into cDNA with the Evo M-MLV RT Mix Kit (Accurate Biotechnology, Changsha, China).

    Article Title: Lactiplantibacillus plantarum LPPerfectus001 Alleviating Irritable Bowel Syndrome in Mice by Modulating Gut Microbiota and NF-κB Signaling Pathway.
    Article Snippet: After this pretreatment, 10 μL of a 100 μg/mL LPS (Sigma-Aldrich, Natick, MA, USA) working solution was added, and the cells were incubated for an additional 22 h. After treatment, both the cells and supernatants were collected. .. The concentration of nitric oxide (NO) in the supernatant was measured using Nitric Oxide (NO) Colorimetric Assay Kit (Elabscience, Wuhan, China). .. To determine the expression level of pro- inflammatory genes, total RNA was isolated using AG RNAex Pro reagent and reverse-transcribed into cDNA with the Evo M-MLV RT Mix Kit (Accurate Biotechnology, Changsha, China).

    Colorimetric Assay:

    Article Title: Lactiplantibacillus plantarum LPPerfectus001 Alleviating Irritable Bowel Syndrome in Mice by Modulating Gut Microbiota and NF-κB Signaling Pathway
    Article Snippet: After this pretreatment, 10 μL of a 100 μg/mL LPS (Sigma-Aldrich, Natick, MA, USA) working solution was added, and the cells were incubated for an additional 22 h. After treatment, both the cells and supernatants were collected. .. The concentration of nitric oxide (NO) in the supernatant was measured using Nitric Oxide (NO) Colorimetric Assay Kit (Elabscience, Wuhan, China). .. To determine the expression level of pro- inflammatory genes, total RNA was isolated using AG RNAex Pro reagent and reverse-transcribed into cDNA with the Evo M-MLV RT Mix Kit (Accurate Biotechnology, Changsha, China).

    Article Title: Protective effects of Allium sativum essential oil against lead nitrate-induced cardiotoxicity: Modulation of lipid metabolism, nitric oxide dynamics, inflammatory mediators, and histological profiles in Swiss albino mice
    Article Snippet: This evaluation involved the measurement of total cholesterol (TC) , while low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were quantified using the Elabscience kits (Catalog No: E-EL-M1363) and (Catalog No: E-EL-M1402), respectively. .. Nitric oxide (NO) levels were indirectly quantified by measuring nitrite (NO2−) concentrations using the Nitric Oxide (NO) Colorimetric Assay Kit (Elabscience, Catalog No: E-BC-K035-M). ..

    Article Title: Microglial GLUL loss worsens TBI outcomes by amplifying the arginine-citrulline pathway.
    Article Snippet: Microglial hyperactivation-induced neuroinflammation is a central driver of neurological dysfunction after traumatic brain injury (TBI).. Metabolic reprogramming is essential for microglial activation, but the specific metabolic alterations following TBI and their causal relationship with activation remain poorly defined.. This study investigated the role and underlying mechanisms of glutamate-ammonia ligase (GLUL) in regulating microglial activation after TBI.

    Article Title: Evaluations of the intracellular activity of glycoproteins extracted from kiwifruit: Repair of oxidative damage and immunoregulation in hydrogen peroxide-treated HepG2 cells and RAW264.7 macrophages
    Article Snippet: A glycoprotein (KGP) was isolated from kiwifruit using enzyme extraction.. Structural characterization revealed that KGP, with a molecular weight of 79 kDa, is a high-purity filamentous glycoprotein containing 81.41 % protein and 11.15 % total carbohydrate.. In vitro cellular assays demonstrated that KGP can enhance mitochondrial membrane potential and reduce cellular apoptosis rates.

    Article Title: Cerebellar GluN2C protects against sleep deprivation-induced cognitive impairment and motor incoordination.
    Article Snippet: Sleep loss, particularly long-term sleep insufficiency or clinical sleep disorders, is a global health problem that affects millions of people and has been linked to accelerated cognitive decline and increased dementia risk.. The cerebellum, traditionally viewed as a motor-control center, is now recognized to modulate emotion, cognition and sleep.. Emerging evidence indicates that the cerebellum exhibits sleep stage-dependent activity and is closely associated with learning and memory processes.

    Article Title: Lactiplantibacillus plantarum LPPerfectus001 Alleviating Irritable Bowel Syndrome in Mice by Modulating Gut Microbiota and NF-κB Signaling Pathway.
    Article Snippet: After this pretreatment, 10 μL of a 100 μg/mL LPS (Sigma-Aldrich, Natick, MA, USA) working solution was added, and the cells were incubated for an additional 22 h. After treatment, both the cells and supernatants were collected. .. The concentration of nitric oxide (NO) in the supernatant was measured using Nitric Oxide (NO) Colorimetric Assay Kit (Elabscience, Wuhan, China). .. To determine the expression level of pro- inflammatory genes, total RNA was isolated using AG RNAex Pro reagent and reverse-transcribed into cDNA with the Evo M-MLV RT Mix Kit (Accurate Biotechnology, Changsha, China).

    Article Title: Icariin improves erectile function in spontaneously hypertensive rats by downregulating GRK2 in penile cavernous tissue.
    Article Snippet: Background: Hypertension is an independent risk factor for erectile dysfunction (ED).. Icariin can improve erectile function of spontaneous hypertensive rats (SHRs).. GRK2 is closely related to the phosphorylation of eNOS and endothelial function.

    Article Title: Cognitive and psychotic effects of ketamine "short- vs. long-term" therapy in a rat model of depression: Hippocampal TrkB/Akt/GSK-3β/mTOR/autophagy trajectories.
    Article Snippet: Accumulating evidence indicates the role of ketamine as a rapid-onset antidepressant, with a rising controversy regarding its cognitive and psychotic effects, especially with long-term use.. However, no previous experimental study has directly compared short-term and long-term ketamine effects using a time schedule that simulates infusion protocols in major depressive disorder patients.. This study investigated the shortversus long-term antidepressant, cognitive, and psychotic effects of ketamine (10 mg/kg), with an emphasis on hippocampal mechanistic cascades in a chronic unpredictable stress (CUS) rat model of depression.

    In Vivo:

    Article Title: Microglial GLUL loss worsens TBI outcomes by amplifying the arginine-citrulline pathway.
    Article Snippet: Microglial hyperactivation-induced neuroinflammation is a central driver of neurological dysfunction after traumatic brain injury (TBI).. Metabolic reprogramming is essential for microglial activation, but the specific metabolic alterations following TBI and their causal relationship with activation remain poorly defined.. This study investigated the role and underlying mechanisms of glutamate-ammonia ligase (GLUL) in regulating microglial activation after TBI.

    BIA-KA:

    Article Title: Evaluations of the intracellular activity of glycoproteins extracted from kiwifruit: Repair of oxidative damage and immunoregulation in hydrogen peroxide-treated HepG2 cells and RAW264.7 macrophages
    Article Snippet: A glycoprotein (KGP) was isolated from kiwifruit using enzyme extraction.. Structural characterization revealed that KGP, with a molecular weight of 79 kDa, is a high-purity filamentous glycoprotein containing 81.41 % protein and 11.15 % total carbohydrate.. In vitro cellular assays demonstrated that KGP can enhance mitochondrial membrane potential and reduce cellular apoptosis rates.

    Multiple Displacement Amplification:

    Article Title: Cognitive and psychotic effects of ketamine "short- vs. long-term" therapy in a rat model of depression: Hippocampal TrkB/Akt/GSK-3β/mTOR/autophagy trajectories.
    Article Snippet: Accumulating evidence indicates the role of ketamine as a rapid-onset antidepressant, with a rising controversy regarding its cognitive and psychotic effects, especially with long-term use.. However, no previous experimental study has directly compared short-term and long-term ketamine effects using a time schedule that simulates infusion protocols in major depressive disorder patients.. This study investigated the shortversus long-term antidepressant, cognitive, and psychotic effects of ketamine (10 mg/kg), with an emphasis on hippocampal mechanistic cascades in a chronic unpredictable stress (CUS) rat model of depression.



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